Trump Education Department launches Title IX probes into 2 blue states allegedly ignoring trans athlete order

That came just days after the California Interscholastic Federation (CIF) said it will continue to follow the state's law that allows athletes to participate as whichever gender they identify as.

"The Minnesota State High School League and the California Interscholastic Federation are free to engage in all the meaningless virtue-signaling that they want, but at the end of the day they must abide by federal law," said Craig Trainor, acting assistant secretary for civil rights. 

"(The Office of Civil Rights') Chicago and San Francisco regional offices will conduct directed investigations into both organizations to ensure that female athletes in these states are treated with the dignity, respect and equality that the Trump administration demands. I would remind these organizations that history does not look kindly on entities and states that actively opposed the enforcement of federal civil rights laws that protect women and girls from discrimination and harassment."

The Department of Education added that state laws do not override federal anti-discrimination laws, so the associations are subject to investigations.

The order states that "it is the policy of the United States to rescind all funds from educational programs that deprive women and girls of fair athletic opportunities" and to take "all appropriate action to affirmatively protect all-female athletic opportunities and all-female locker rooms and thereby provide the equal opportunity guaranteed by Title IX of the Education Amendments Act of 1972."

Prior to Trump signing the order Wednesday, White House press secretary Karoline Leavitt said part of the motivation behind Trump's executive order would be to create a "pressure campaign" for the International Olympic Committee (IOC) and NCAA to follow and prevent transgender athletes from competing in women's sports. 

In response to Trump's order, the NCAA changed its policy, in place since 2010, to disallow transgender athletes from competing against women, requiring them to compete based on their birth gender.

Fox News' Jackson Thompson and The Associated Press contributed to this report.